Monitor and control your upstream bioprocessing using a deployable MS

The new demands of bioprocessing, used to make biologics, means that there is a need for analytical instruments, such as mass spectrometers, to provide real­time information (on-line and at-line) at the point-of-need. We show how our deployable, and compact mass spectrometer sam­pling the media from a bioreactor in real-time could provide important process monitoring, and critical quality attributes of the product.

Deployable mass detection integrated into your process can enhance productivity and profitability

Microsaic Systems‘ application portfolio ranges from traditional LC-MS analysis to previously unserved markets that will benefit from deployable mass detection at the point-of-need. In this application note the versatility of Microsaic Systems’ application range is detailed along with the benefits of enhanced process productivity and profitability by integrating simple-to-use mass detection.

Point-of-need mass spectrometry for the monitoring of metabolites and IgG in upstream bioprocessing

The cost, size and complexity of mass spectrometry instrumentation has previously limited the use of it to centralised laboratories. This App Note shows how the latest miniaturised mass spectrometers (ESI-MS) allow the monitoring of both metabolites and IgG in upstream cell cultures, and at the point-of-need.

Using mass spectrometry for point-of-need bioprocessing

Integrated, real-time compact mass spectrometry provides important process information quickly, and at the point-of-need. This App Note discusses the analysis of storage time and conditions on monoclonal antibodies using miniaturised mass spectrometers.

Easy and fast isolation of rosmarinic acid from lemon balm with mass-directed purification

Rosmarinic acid is a natural product widely spread over different plant families. Preparative reversed-phase chromatography was used for the mass-directed purification of rosmarinic acid from a lemon balm extract.

Sensitive Detection of Peptides in Cytochrome C Digest Using Nano LC with Micro Pillar Array Columns (µPACTM) and Microsaic 4500 MiD® Mass Spectrometer

Liquid Chromatography coupled with mass spectrometry is a powerful analytical tool for detection and identification of chemicals in complex mixtures. The compact nature of the Microsaic 4500 MiD® MS system and the combination of low flow rate requirements and high separation resolution that PharmaFluidics µPAC™ LC columns can offer have made this combination a perfect fit for performing targeted proteomics studies as shown in this application note.

Rapid Analysis of Upstream Bioprocesses Using a Point-of-Need Mass Spectrometer

Integrated real-time MS, decentralized and at the point-of-need, could provide important process information quickly and at reduced cost. Here, the feasibility of using a deployable and compact mass spectrometer for the direct analysis of upstream cell cultures is investi­gated.

Purification of epigallocatechin gallate and other related polyphenols from green tea by mass-triggered fractionation

In collaboration with Knauer, this Application Note demonstrates an easy time-saving method for the isolation of polyphenolic compounds in green tea extract based on a technique of mass-triggered fractionation with HPLC and miniaturised mass spectrometer.

Rapid detection of counterfeit and adulterated alcoholic drinks by deployable and direct ESI-MS

Using at-line direct injection MS analysis, we have demonstrated the ability to distinguish not only a difference in brands of whisky from the same distillery, but a detectable difference in different batches of the same blend. Furthermore, a test to detect the presence of denaturants or additives in whisky has been devised.

Investigating polar phenolic compounds in peppermint rhizomes and leaves using 4500 MiD®

Peppermint’s reported biological activities, including digestion-stimulation and antiseptic effects, are thought to be influenced by naturally-occurring polar phenolic compounds. This App Note shows how compact mass spectrometry can be used to quickly identify and quantify key compounds in different parts of the plant.

Application of high performance microfluidic electrokinetic liquid chromatography-mass spectrometry (eHPLC-MS) in separation and analysis of isomers of microcystins

Separation and analysis based on high performance microfluidic electrokinetic liquid chromatography (eHPLC) coupled with mass spectrometry gives multiple analytical benefits including high sensitivity and high selectivity. This App Note shows how Microsaic’s benchtop quadrupole mass spectrometer is easily deployed in eHPLC-MS analysis, and it provides fast, information-rich data about the test substance.

Cannabinoids and TLC-MS

The use of cannabis and cannabis derivatives in medicine and nutritional supplements has now grown internationally, largely due to favourable regulatory status for medicinal use. As more new compounds are released onto the market, accessible techniques are required to quickly and efficiently analyse product quality.

Direct TLC-MS analysis using the 4000 MiD

TLC-MS system provides an easy way to identify and elucidate unknown substances in research, forensic and environmental fields. Integration of the TLC-MS interface with the miniaturised, deployable, single quadrupole 4000MiD® mass detector provides a completely standalone and easy to use solution for TLC spot identification.

At-line extraction MS analysis of caffeine in Redbull

We report a rapid and low cost screening of caffeine in RedBull by using a hand-held automated extraction platform coupled to the 4000 MiD® mass detector.

Purification of peptides and small molecules by Prep-LCMS

Preparative liquid chromatography (Prep-LC) has become a major tool in the production of specialty chemicals. Prep-LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. This technique allows collecting fractions with high purity and excellent recovery for target molecules by combining ultra violet (UV) and mass-based detection.

Multivariate analysis of a SNAr by on-line reaction monitoring using miniaturized MS

Investigation of the optimization of the nucleophilic aromatic substitution of 2,4-difluoro-nitrobenzene with morpholine over a wide variety of stochiometric ratios, residence times and temperatures to demonstrate all the benefits of using on-line flow reaction monitoring.

HPLC-MS applications of the Microsaic 4000 MiD®

Here we report the HPLC-MS analysis of pesticides, peptides, carbohydrates and ibuprofen to demonstrate the excellent versatility and simplicity of the Microsaic 4000 MiD® in several areas of application, such as environmental monitoring, food safety, forensics and pharmaceuticals.

Bromination of a pyrrolo-pyrimidine by real-time flow reaction monitoring using MS analysis

Report on the collaboration with Durham University using the 4000 MiD® mass spectrometer to demonstrate the benefits of on-line flow reaction monitoring of the bromination of ethyl pyrrolo[1,2-c]pyrimidine-3-carboxylate.

Batch monitoring of deprotection chemistry

Here we monitor and compare two Boc-deprotection reactions using Microsaic Systems unique mass detection and sampling platform, consisting of the MiDasTM, 4000 MiD® and stand-alone on-board Masscape® software.

On-line flow reaction monitoring of Wittig reaction using deployable mass detection

Syrris and Microsaic Systems, both R&D 100 award winning companies have set up a Flow reactor-MS system to demonstrate the benefits of using on-line flow reaction monitoring. This presents a revolutionary tool for organic chemistry synthesis.

On-line MS using the 4000 MiD reported by Astra-Zeneca at BMSS 2013

Report on the use of the 4000 MiD® mass spectrometer for on-line monitoring of continuous flow chemical synthesis – the Hofmann Rearrangement reaction.

Development of a new analytical tool for biologics manufacturing

The MiDex is a small point-of-need solution to easily measure titre and Critical Quality Attributes (CQAs) of monoclonal antibody (mAb) biologic medicines.

Analysis of Perfluorinated Alkyl Substances (PFAS) in River Water Samples Using Online SPE-LC-MS

PFAS or ‘forever chemicals’ bio-accumulate in our environment and in the human body. This application note showcases our portable online detector for PFAS, a solution that can bring the lab to the sample.

Product development and beyond

The 4500 MiD® combines the vacuum system, electronics and computer inside one box.

Concept to Manufacture services of micro-engineered MEMS devices

Access a team of world experts in MEMs technology, create turnkey solutions for any process, and receive ongoing support by outsourcing your R&D projects.

The use of the miniaturised Microsaic 4500 MiD® mass spectrometer as a point-of-need analyser for Process Analytical Technology (PAT) in automated bioprocessing

The small footprint of the Microsaic Metabolite Monitoring PAT system enables measurements to be undertaken at the point-of-need, away from centralised laboratories, allowing real time monitoring.

On-line batch reaction monitoring of the catalytic conversion of ethanol to an advanced biofuel

Report on the collaboration with Bristol University using the 4000 MiD® mass spectrometer to demonstrate the benefits of on-line reaction monitoring for reactions in batch mode of the catalytic conversion of ethanol to an advanced biofuel.

Poster: Sensitive detection of peptides in cytochrome C digest using a real-time compact mass spectrometer

This poster describes a Nano LC-MS analysis of a cytochrome C digest using a micro pillar array column and the Microsaic 4500 MiD, as compared to a UV detector.

Poster: The development of a point-of-need miniaturized mass spectrometer for upstream bioprocessing applications

A point-of-need miniaturized mass spectrometer, in conjunction with Proof-of-Concept (PoC) workflows, have been developed to analyse cell culture samples obtained from a bioreactor.

Poster: Mixed Computational Fluid Dynamics and Direct Simulation Monte Carlo model of the intermediate pressure regions of a miniaturised mass spectrometer

This poster explores the development of a Simion ion trajectory model for a MEMS-based compact mass spectrometer.